The Eberswalde zoological garden plays an active role in the conservation of species under the Convention of International Trade in Endangered Species of Wild Fauna and Flora (CITES). With a highly diverse animal population, it also functions as a living museum of natural history and as an educational and research institution. All areas of the zoo inform visitors about interaction between nature, animals and human beings.

 Nowadays, zoos function as coordination centres for nature and species conservation. Zoos have developed breeding strategies for many endangered species that include binding directives for the breeder. Eberswalde Zoo is taking part in the European Endangered Species Programme (EEP). The aim of this programme is to secure the existence of selected, highly endangered species for the future.

 Eberswalde Zoo is the only zoo in Brandenburg that is a member of the Species Conservation Foundation. The foundation is a joint initiative, currently comprising of 45 respected zoos, animal parks and nature conservation organisations. The foundation aims to preserve animal species which, although under severe threat of global extinction, do not have a strong enough lobby for their conservation.